hek293 human embryonic kidney derived cells  (ATCC)

Journal: Discover Nano

Article Title: Novel loading protocol combines highly efficient encapsulation of exogenous therapeutic toxin with preservation of extracellular vesicles properties, uptake and cargo activity

doi: 10.1186/s11671-024-04022-8

Figure Lengend Snippet: Evaluation of structural modifications of nsEVs after sodium carbonate treatment. Transmission electron microscopy (TEM) image ( A ) and nanoparticle tracking analysis ( B ) of HEK293-derived nsEVs after sodium carbonate treatment. Western blot analysis ( C ) of Alix, TSG101, CD63 and CD9 before (pre) and after (post) sodium carbonate treatment. Comparison of nsEVs treated with phosphate buffer saline (PBS) or sodium carbonate (Na 2 CO 3 ) through silver staining ( D ) single-particle interferometric reflectance imaging sensor (SP-IRIS) analysis (E) and Raman spectroscopy (F). M: molecular weight marker

Article Snippet: HEK293 (human embryonic kidney-derived) cells were purchased from ATCC.

Techniques: Transmission Assay, Electron Microscopy, Derivative Assay, Western Blot, Comparison, Saline, Silver Staining, Single Particle, Imaging, Raman Spectroscopy, Molecular Weight, Marker

Journal: Discover Nano

Article Title: Novel loading protocol combines highly efficient encapsulation of exogenous therapeutic toxin with preservation of extracellular vesicles properties, uptake and cargo activity

doi: 10.1186/s11671-024-04022-8

Figure Lengend Snippet: Encapsulation efficiency evaluation and comparison between electroporation and sodium carbonate treatment. A Spectrofluorometer analysis for quantification of Dextran-FITC incorporation after 50 V electroporation (see material and methods) or sodium carbonate treatment of HEK293-derived nsEVs (25 µg/ml) in various nsEVs:Dextran-FITC ratios (1:1, 1:1.5 and 1:2). B Confocal microscopy images of Hela cells treated for 24 h with Dextran-FITC bearing nsEVs (25 µg/ml) after electroporation (50 V) or sodium carbonate incubation in NsEVs:Dextran-FITC = 1:1.5 molecular ratio (blue = DAPI; green = Dextran-FITC bearing nsEVs). C Spectrofluorometer analysis for quantification of SAP-FITC incorporation after 50 V electroporation (see material and methods) or sodium carbonate treatment of HEK293-derived nsEVs (25 µg/ml) in nsEVs:SAP-FITC = 1:1.5 ratio

Article Snippet: HEK293 (human embryonic kidney-derived) cells were purchased from ATCC.

Techniques: Encapsulation, Comparison, Electroporation, Derivative Assay, Confocal Microscopy, Incubation

Journal: Discover Nano

Article Title: Novel loading protocol combines highly efficient encapsulation of exogenous therapeutic toxin with preservation of extracellular vesicles properties, uptake and cargo activity

doi: 10.1186/s11671-024-04022-8

Figure Lengend Snippet: Evaluation of sodium carbonate-treated SAP-bearing nsEVs uptake. A Bright field (upper row) and fluorescent (lower row) microscopy images of 5637 cells 24 h after incubation with HEK293-derived nsEVs treated with phosphate buffer saline (PBS, left) or sodium carbonate (Na 2 CO 3 , right) in the presence of SAP and stained with Vybrant Dio lipophilic dye. Different concentrations of nsEVs (5 and 10 µg) were used for comparison. Representative images of three independent experiments. B Flow cytometry analysis of 5637 cells 24 h after treatment with 5 or 10 µg of PBS or Na 2 CO 3 -treated SAP-bearing nsEVs. Results are shown as mean fluorescence percentage + SD from 3 independent experiments

Article Snippet: HEK293 (human embryonic kidney-derived) cells were purchased from ATCC.

Techniques: Microscopy, Incubation, Derivative Assay, Saline, Staining, Comparison, Flow Cytometry, Fluorescence

Journal: Journal of Materials Science

Article Title: A hierarchically porous and SLIT3-releasing scaffold for bone tissue engineering applications

doi: 10.1007/s10853-024-10379-z

Figure Lengend Snippet: Figure 10 SLIT3-loaded and SLIT3-free scaffold cell population after different time period at three cell densities.

Article Snippet: Human embryonic kidney cell (HEK293)-derived SLIT3 recombinant protein with C-terminal 6-his-tag and a predicted molecular mass of 45 kDa was purchased from R&D systems, USA.

Techniques:

Journal: Cell Reports Medicine

Article Title: A diverse collection of B cells responded to HIV infection in infant BG505

doi: 10.1016/j.xcrm.2021.100314

Figure Lengend Snippet:

Article Snippet: For antibody production: HEK293-F cells (RRID:CVCL_D603; originally derived from female human embryonic kidney cells) were obtained from Invitrogen (Thermo Fisher Scientific, Waltham, MA, catalog #R790-07) and grown at 37°C in Freestyle 293 Expression Medium (Thermo Fisher Scientific, catalog #12338002) in baffle-bottomed flasks orbiting at 135 rpm.

Techniques: Clone Assay, Recombinant, Expressing, Sequencing, Amplification, Plasmid Preparation, Software